- Cell Line: NCI-H1618
- Synonyms:
- NCI-H618
- NCIH1618
- H1618
- H-1618
- Culture Type: Cell line
- Keywords: Human Lung Carcinoma Cell Line
- Description: Established from the lungs of a 55-year-old, Caucasian, female patient with small cell lung cancer (carcinoma), stage E
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 51 years
- Ethnicity: Caucasian
- Gender: Female
- Smoking habit:-
- Tissue (origin): Lung
- Disease: Small cell lung cancer (carcinoma), stage E
- Primary/Metastasis: Metastatic
- Collection Site: Bone marrow
- Year of origin: 1987
- Organism:
- Cell line features:
- Cell Type:-
- Morphology:
- Growth mode: Suspension, multicellular aggregates
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Microsatellite instability:-
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- TP53 (R248L mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000179?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000179?tab=fusions
- TP53 (R248L mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM:F12 Medium supplemented with
- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite
- 10 nM Hydrocortisone
- 10 nM beta-estradiol
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time:-
- Seeding density:-
- Recommended split ratio:-
- Medium Renewal: Add fresh medium every 3 to 4 days
- Subculturing method: Dilution
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% (v/v) FBS + 7.5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% (v/v) FBS + 7.5% (v/v) DMSO
- Applications:
- Cancer research
- 3D cell culture
- Immunology
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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