- Cell Line: NCI-H1703
- Synonyms:
- NCI-H1703
- NCIH1703
- H1703
- H-1703
- Culture Type: Cell line
- Keywords: Human Lung Squamous Cell Carcinoma Epithelial Cell line
- Description: Established from the lungs of a 54-year-old Caucasian male with a lung squamous cell carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 54 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Disease:
- Non-Small Cell Lung Cancer
- Disease subtype: Lung Squamous Cell Carcinoma
- Molecular subtype: NSCLC Squamous
- Primary/Metastasis: Primary
- Non-Small Cell Lung Cancer
- Year of origin: 1987
- Organism:
- Cell line features:
- Cell Type:
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time: 47.9 hours
- Microsatellite instability: Stable
- Special features:
- Product:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- CREBBP (W1472C mutation, Likely Oncogenic, Unknown)
- For information, please visit
- Cell Type:
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:
- Seeding density:
- Split ratio: 1:3 to 1:6
- Medium Renewal: Two to three times weekly
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO (ATCC)
- Culture medium + 50% FBS + 10% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- NCI-H1703 cell image link (Source: DepMap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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