Cell Line: NCI-H1963

  • Cell Line: NCI-H1963
  • Synonyms:
    • NCI-H1963
    • NCIH1963
    • H1963
    • H-1963
  • Culture Type: Cell line
  • Keywords: Human Lung Small Cell Carcinoma Cell line
  • Description: Established from the lungs of a 56-year-old, Black male patient with stage L small cell lung carcinoma
  • Origin:
    • Organism: 
      • Species: Human (Homo sapiens)
      • Age: 56 years
      • Ethnicity: Black
      • Gender: Male
    • Tissue (origin): Lung
    • Disease: Small cell lung carcinoma, stage L
    • Primary/Metastasis: Primary
    • Year of origin: 1988
  • Cell line features:
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium:
      • RPMI-1640 Medium + 10% FBS (ATCC)
      • RPMI-1640 Medium + 10% heat-inactivated FBS (DSMZ)
    • Culture conditions:
      • Temperature: Humidified 37°C incubator
      • Atmosphere: 5% CO2, 95% Air
    • Subculturing:
      • Subculturing method: Dilution (addition/replacement of medium)
      • Harvesting time: When cell density reaches 1-2 x 106 cells/ml (DSMZ)
      • Seeding density: 0.5 x 106 cells/ml (DSMZ)
      • Split ratio: 1:2 (DSMZ)
      • Split frequency: every 3 days (DSMZ)
      • Medium Renewal:-
    • Cryopreservation:
      • Freezing medium:
        • Complete growth medium + 5% DMSO (ATCC)
        • Complete growth medium + 20% FBS + 10% DMSO (DSMZ)
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial:
  • Applications: 
    • Cancer research
    • 3D cell culture
  • Availability:
    • American Type Culture Collection (ATCC): Product link 
    • DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
  • References
  • Images:
  • Abbreviations
    • BSL (Biosafety level)
    • FBS (Fetal Bovine Serum)
    • DMSO (Dimethyl sulfoxide)
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