- Cell Line: NCI-H2009
- Synonyms:
- NCI-H2009
- NCIH2009
- H2009
- H-2009
- Culture Type: Cell line
- Keywords: Human Lung Adenocarcinoma Cell Line
- Description: Established from the lungs of a 68-year-old, Caucasian, female patient with stage 4 adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 68 years
- Ethnicity: Caucasian
- Gender: Female
- Smoking habit: smoker
- Tissue (origin): Lung
- Disease: Non-Small Cell Lung Cancer, stage 4 (Lung Adenocarcinoma)
- Primary/Metastasis: Metastatic (lymph_node)
- Year of origin: 1988
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time: 29.8 hours
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- TP53 (R273L mutation, Likely Oncogenic, Loss-of-function, Homozygous)
- KRAS (G12A mutation, Oncogenic, Gain-of-function, Heterozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000886?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000886?tab=fusions
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM:F12 Medium supplemented with
- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite
- 10 nM Hydrocortisone
- 10 nM beta-estradiol
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:3 to 1:6
- Medium Renewal: Two to three times weekly
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 10 % (v/v) FBS + 5 % (v/v) DMSO
- Complete growth medium + 10 % (v/v) FBS + 5 % (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Cancer research
- Availability:
- American Type Culture Collection (ATCC): Product link
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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