- Cell Line: NCI-H2106
- Synonyms:
- NCI-H2106
- NCIH2106
- H2106
- H-2106
- Culture Type: Cell line
- Keywords: Human Non-small Cell Lung Carcinoma Cell line
- Description: Established from the Lymph node (metastatic site) of a 58-year-old, caucasian male patient with stage 4 non-small cell lung carcinoma.
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 58 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Lymph node
- Disease: Non-small cell lung carcinoma, stage 4
- Disease subtype: Large Cell Lung Carcinoma
- Molecular subtype: NSCLC Large Cell
- Primary/Metastasis: Metastatic
- Year of origin: 1988
- Organism:
- Cell line features:
- Cell Type:-
- Morphology:-
- Growth mode: Suspension, floating clusters
- Life span: Infinite (continuous culture)
- Karyotype:-
Doubling time:- - Microsatellite instability:-
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM:F12 (ATCC, Catalog No.30-2006) + Supplements
- Supplements
- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite (final conc.)
- 10 nM Hydrocortisone (final conc.)
- 10 nM beta-estradiol (final conc.)
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum (final conc.)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution (addition/replacement of medium)
- Harvesting time:-
- Seeding density:-
- Split ratio:-
- Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
- Subculturing method: Dilution (addition/replacement of medium)
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% FBS + 5% DMSO
- Complete growth medium + 5% FBS + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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