- Cell Line: NCI-H292
- Synonyms:
- NCI-H292
- NCIH292
- H292
- H-292
- NCI-HUT-292
- Hut292
- Culture Type: Cell line
- Keywords: Human pulmonary mucoepidermoid carcinoma cell line
- Description: Established from lymph node metastasis of a pulmonary mucoepidermoid carcinoma of the lung from a 32 year-old black female
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 32 years
- Ethnicity: Black
- Gender: Female
- Smoking habit:-
- Tissue (origin): Lung
- Disease: Mucoepidermoid Carcinoma of the Lung (LUMEC)
- Primary/Metastasis: Metastatic (Collection Site: lymph node)
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: Epithelial
- Morphology: Epithelial-like
- Growth mode: Adherent, grown as monolayer
- Life span: Infinite (continuous culture)
- Karyotype: near-diploid, modal chromosome number 47
Doubling time: Approximately 48 hrs - Microsatellite instability: Instable
- Special features:
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity: yes, produce tumors in nude mice
- Gene expression:
- mucicarmine positive
- keratin positive
- vimentin positive
- negative for neurofilament triplet protein
- L-DOPA decaroxylase negative
- Sequence variations:
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-001075?tab=mutations
- https://depmap.org/portal/cell_line/ACH-001075?tab=fusions
- For information, please visit
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time: Semi confluent culture (~ 80%)
- Seeding density:-
- Recommended split ratio: 1:3 to 1:8
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO??
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% DMSO??
- Applications:
- Cancer research
- 3D cell culture
- High-throughput screening
- Toxicology
- Virus replication studies (Hepatitis B virus)
- Availability:
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- NCI-H292 cell image link (Source: DepMap)
- NCI-H292 cell image link 1 (Source: Cytion)
- NCI-H292 cell image link 2 (Source: Cytion)
- NCI-H292 cell image link 3 (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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