- Cell Line: NCI-H508
- Synonyms:
- NCI-H508
- NCIH508
- H508
- H-508
- Culture Type: Cell line
- Keywords: Human Colorectal Adenocarcinoma Cell line
- Description: Established from the abdominal wall metastasis of a Caucasian, 55-year-old, male patient with colorectal adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 55 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Large intestine, Cecum
- Tissue (isolation): Abdominal wall
- Disease:
- Colorectal Adenocarcinoma
- Primary/Metastasis: Metastatic
- Year of origin:
- Organism:
- Cell line features:
- Cell Type:
- Morphology: Round cells/Epithelial-like
- Growth mode: Mixed: floating aggregates of round cells with some attached cells
- Life span: Infinite (continuous culture)
- Karyotype: Modal number = 102; range = 71 to 131
Doubling time: 32 hours - Microsatellite instability: Stable
- Special features:
- Product:-
- Tumorigenicity: Produce tumors in nude mice
- Gene expression:-
- Cells express carcinoembryonic antigen (CEA)
- Antigen
- Express Blood type A, Rh, CA19-9
- Do not express the TAG-72 antigen
- Sequence variations:
- TP53 (R273H mutation, Oncogenic, Loss-of-function, Homozygous)
- SPOP (E47K mutation, Likely Oncogenic, Loss-of-function)
- PIK3CA (E545K mutation, Oncogenic, Gain-of-function, Heterozygous)
- BRAF (G596R mutation, Oncogenic Gain-of-function, Heterozygous)
- For information, please visit
- Cell Type:
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation + harvesting floating cells by centrifugation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Split ratio: 1:2 to 1:4
- Medium Renewal: 3 times per week
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- Cytion: Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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