- Cell Line: NCI-H647
- Synonyms:
- NCI-H647
- NCIH647
- H647
- H-647
- Culture Type: Cell line
- Keywords: Human Lung Adenosquamous Carcinoma Cell Line
- Description: Established from the lungs of a 56-year-old, Caucasian male with adenosquamous carcinoma, stage 3A
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 56 years
- Ethnicity: Caucasian
- Gender: Male
- Smoking habit: non-smoker
- Tissue (origin): Lung
- Disease: Non-small cell lung cancer (Adenosquamous Carcinoma, Stage 3A)
- Primary/Metastasis: Metastatic (DepMap)
- Collection site: Pleural effusion (DepMap)
- Year of origin: 1983
- Organism:
- Cell line features:
- Cell Type:-
- Morphology:-
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: Modal number = 89; range = 74-176; t(3p14;11p15)
- Doubling time:-
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- KRAS (G13D mutation, Oncogenic, Gain-of-function, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000378?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000378?tab=fusions
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% trypsin, 0.03% EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:3 to 1:6
- Medium Renewal: Twice per week
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Cancer research
- Availability:
- American Type Culture Collection (ATCC): Product link
- Cytion: Product link
- References
- Images:
- NCI-H647 cell line image link 1 (Source: DepMap)
- NCI-H647 cell line image link 1 (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
