- Cell Line: NUC-1
- Synonyms:
- Culture Type: Cell line
- Keywords: Mouse Urinary Bladder Cell Line
- Description: Established from urinary bladder of an adult female C3H/Law mouse
- Origin:
- Organism:
- Species: Mouse (Mus musculus)
- Age: Adult
- Breed: C3H/Law
- Gender: Female
- Species: Mouse (Mus musculus)
- Tissue (origin): Urinary bladder
- Disease:-
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Spindle cell-like, disorderly arranged cells
- Growth mode: Adherent, growing in multilayers
- Life span: Infinite (continuous culture)
- Karyotype: Hypotetraploid flat-moded murine karyotype
- Doubling time: ~ 24 hours
- Special features: in vitro immortalized
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity: Yes
- Gene expression:
- Sequence variations:
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: 45% Ham’s F10 + 45% DMEM + 10% h.i. FBS + 5 µg/ml human insulin + 5 µg/ml human transferrin + 50 nM hydrocortisone + 5 ng/ml selenite + 10 µM HEPES
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: Trypsin/EDTA solution
- Harvesting time:-
- Seeding density:
- Recommended split ratio: 1:10
- Medium Renewal:-
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO
- Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): 2 x 106 cells/ml
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Availability:
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- References
- Images:
- NUC-1 cell line image link 1 (Source: DSMZ)
- NUC-1 cell line image link 2 (Source: DSMZ)
- NUC-1 cell line image link 3 (Source: DSMZ)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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