- Cell Line: SK-MEL-2
- Synonyms:
- SK-MEL-2
- SKMEL-2
- SKMEL2
- SKMel2
- SK-Mel2
- SK Mel 2
- Sk-Mel-2
- Culture Type: Cell line
- Keywords: Human Melanoma Cell Line
- Description: Established from skin tissue derived from a 60-year-old, Caucasian, male patient with malignant melanoma
- Origin:
- Cell line features:
- Cell Type:-
- Morphology: Polygonal
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Doubling time: ~ 32 hours
- Karyotype: Hypodiploid to hypertetraploid (ATCC)
- Microsatellite instability: Instable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity: Yes, in nude mice, forms malignant melanoma (ATCC)
- Gene expression:-
- Sequence variations:
- TP53 (G245S mutation, Oncogenic, Loss-of-function, Heterozygous)
- NRAS (Q61R mutation, Oncogenic, Gain-of-function, Homozygous)
- For more information, please visit:
- https://depmap.org/portal/cell_line/ACH-001190?tab=mutations
- https://depmap.org/portal/cell_line/ACH-001190?tab=fusions
- https://depmap.org/portal/cell_line/ACH-001190?tab=translocations
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Eagle’s Minimum Essential Medium + 10% FBS (ATCC)
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.03 mM EDTA solution (ATCC)
- Harvesting time:
- Seeding density:-
- Split ratio: 1:3 to 1:6
- Split frequency:-
- Medium Renewal: 2 to 3 times per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Applications:
- 3D cell culture
- Cancer research
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- Cytion: Product link
- References
- Images:
- SK-MEL-2 cell line image link 1 (Source: Cytion)
- SK-MEL-2 cell line image link 2 (Source: Cytion)
- SK-MEL-2 cell line image link 3 (Source: Cytion)
- SK-MEL-2 cell line image link 4 (Source: Cytion)
- SK-MEL-2 cell line image link 5 (Source: Cytion)
- SK-MEL-2 cell line image link 6 (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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