- Cell Line: SK-MEL-30
- Synonyms:
- SK-MEL-30
- SKMEL-30
- SKMEL30
- SKMel30
- SK-Mel30
- SK Mel 30
- Sk-Mel-30
- Culture Type: Cell line
- Keywords: Human Melanoma Cell Line
- Description: Established from skin tissue derived from a 67-year-old, Caucasian, male patient with malignant melanoma
- Origin:
- Cell line features:
- Cell Type: Melanoma (DSMZ)
- Morphology: Epithelial-like
- Growth mode: Adherent, monolayer
- Life span: Infinite (continuous culture)
- Doubling time: ~ 30 hours (DSMZ)
- Karyotype: Hypertriploid karyotype with 3.5% polyploidy
- Microsatellite instability: Stable
- Special features: Pigmented
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- TP53 (R273H mutation, Oncogenic, Loss-of-function)
- NRAS (Q61K mutation, Oncogenic, Gain-of-function, Heterozygous)
- BRAF (D287H mutation, Oncogenic, Gain-of-function)
- BRAF (E275K mutation, Likely Oncogenic, Likely Gain-of-function
- For more information, please visit:
- https://depmap.org/portal/cell_line/ACH-000810?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000810?tab=fusions
- Cell Type: Melanoma (DSMZ)
- Handling and maintenance:
- Biosafety level: BSL 1 (DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI 1640 + 10% heat-inactivated FBS (DSMZ)
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: Trypsin- EDTA solution
- Harvesting time:
- Seeding density:-
- Split ratio: 1:4 to 1:10
- Split frequency:-
- Medium Renewal:-
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO (DSMZ)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Applications:
- Cancer research
- Cell Line Availability: The cell line can be obtained from the following sources.
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- References
- Images:
- SK-MEL-30 cell line image link 1 (Source: DSMZ)
- SK-MEL-30 cell line image link 2 (Source: DSMZ)
- SK-MEL-30 cell line image link 3 (Source: DSMZ)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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