- Cell Line: SU-DHL-1
- Synonyms:
- SU-DHL-1
- SUDHL1
- SUDHL-1
- SuDHL-1
- SuDHL 1
- Stanford University-Diffuse Histiocytic Lymphoma-1
- Culture Type: Cell line
- Keywords: Human Large Cell Lymphoma Cell Line
- Description: Established from the pleural effusion of a 10- year-old boy with Anaplastic Large-Cell Lymphoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 10 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue: Lymph node
- Disease: Large Cell Lymphoma
- Primary/Metastasis:-
- Collection site: Pleural effusion (DepMap)
- Year of origin: 1973
- Country of Origin:-
- Organism:
- Cell line features:
- Cell Type: Histiocytic cell
- Morphology: Lymphoblast-like
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype: human flat-moded hypertriploid karyotype with 16% polyploidy
- Doubling time: ~ 40-50 hours
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- TP53 (R273H mutation, Oncogenic, Loss-of-function, Heterozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000664?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000664?tab=fusions
- TP53 (R273H mutation, Oncogenic, Loss-of-function, Heterozygous)
- Cell Type: Histiocytic cell
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC, DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- RPMI-1640 Medium + 10% FBS
- RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time: 1.5 x 106 cells/ml
- Seeding density: 0.3 x 106 cells/ml
- Recommended split ratio: 1:2 to 1:6
- Medium Renewal: every 2 to 4 days
- Subculturing method: Dilution
- Cryopreservation:
- Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO (ATCC)
- Applications:
- 3D cell culture
- Immunology
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- References
- Images:
- SU-DHL-1 cell line image link 1 (Source: ATCC)
- SU-DHL-1 cell line image link 2 (Sources: DSMZ)
- SU-DHL-1 cell line image link 3 (Sources: DSMZ)
- SU-DHL-1 cell line image link 4 (Sources: DSMZ)
- SU-DHL-1 cell line image link 5 (Sources: DSMZ)
- SU-DHL-1 cell line image link 6 (Sources: DepMap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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