Posted in Database: Cell Line

Cell Line: SU-DHL-4

   October 3, 2024  Leave a Comment on Cell Line: SU-DHL-4

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  • Cell Line: SU-DHL-4
  • Synonyms:
    • SU-DHL-4
    • SUDHL4
    • SUDHL-4
    • SuDHL-4
    • SuDHL 4
    • Stanford University-Diffuse Histiocytic Lymphoma-4
  • Culture Type: Cell line
  • Keywords:  Human B Cell Lymphoma Cell Line
  • Description: Established from the peritoneal effusion of a Caucasian, 38-year-old, male patient with diffuse large B-cell lymphoma germinal center B-cell type
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Age: 38 years
      • Ethnicity: Caucasian
      • Gender: Male
    • Tissue: Peritoneal effusion
    • Disease: diffuse large B-cell lymphoma germinal center B-cell type
    • Primary/Metastasis: Metastatic (DepMap)
    • Collection site:
    • Year of origin:
    • Country of Origin:-
  • Cell line features:
    • Cell Type: B lymphocyte
    • Morphology: Lymphoblast-like,
    • Growth mode: Single round cells growing in suspension
    • Life span: Infinite (continuous culture)
    • Karyotype: Human hyperdiploid karyotype with 4% polyploidy
    • Doubling time: ~40 hours
    • Microsatellite instability: Stable
    • Special features:
      • Relatively high expression levels of Bax, Bak, AIF
      • High caspase-9 activity
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity:-
    • Gene expression:-
    • Sequence variations:
      • TP53 (R273C mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
      • EZH2 (Y646S mutation, Oncogenic, Likely Gain-of-function, Heterozygous)
      • For information, please visit
        • https://depmap.org/portal/cell_line/ACH-000365?tab=mutations
        • https://depmap.org/portal/cell_line/ACH-000365?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC, DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium:
      • RPMI-1640 Medium + 10% FBS
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Dilution
      • Cell dissociation solution: NA
      • Harvesting time: 8.0 x 105 and 1.0 x 106 cells/ml (ATCC)
      • Seeding density: 1 – 2  x 105 cells/ml (ATCC)
      • Recommended split ratio: 1:2 to 1:6
      • Medium Renewal: every 2 to 4 days
    • Cryopreservation:
      • Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO (ATCC, DSMZ)
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • 3D cell culture
    • Immunology
  • Cell Line Availability: The cell line can be obtained from the following sources.
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