Posted in Database: Cell Line

Cell Line: SU-DHL-5

   October 5, 2024  Leave a Comment on Cell Line: SU-DHL-5

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  • Cell Line: SU-DHL-5
  • Synonyms:
    • SU-DHL-5
    • SUDHL5
    • SUDHL-5
    • SuDHL-5
    • SuDHL 5
    • Stanford University-Diffuse Histiocytic Lymphoma-5
  • Culture Type: Cell line
  • Keywords:  Human B Cell Lymphoma Cell Line
  • Description: Established from the lymph node of a 17-year-old Caucasian female patient with Large Cell Lymphoma
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Age: 17 years
      • Ethnicity: Caucasian
      • Gender: Female
    • Tissue: Lymph node/Lymphoid
    • Disease: Large Cell Lymphoma
    • Primary/Metastasis: Primary (DepMap)
    • Collection site:
    • Year of origin: 1978
    • Country of Origin:-
  • Cell line features:
    • Cell Type: B lymphocyte
    • Morphology: Lymphoblast-like; small, round to polymorph cells
    • Growth mode: Suspension, multicellular aggregates and single cells
    • Life span: Infinite (continuous culture)
    • Karyotype: Hyperdiploid karyotype with 1.5% polyploidy
    • Doubling time: ~40 hours (DSMZ)
    • Microsatellite instability: Stable
    • Special features:-
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity:-
    • Gene expression:-
    • Sequence variations:
      • FOXO1 (S205T mutation, Likely Oncogenic, Unknown)
      • For information, please visit
        • https://depmap.org/portal/cell_line/ACH-000660?tab=mutations
        • https://depmap.org/portal/cell_line/ACH-000660?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC, DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium:
      • RPMI-1640 Medium + 10% FBS
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Dilution
      • Cell dissociation solution: NA
      • Harvesting time: 5 x 105 viable cells/mL (ATCC)
      • Seeding density: 5 x 104 to 1 x 105 viable cells/mL (ATCC)
      • Recommended split ratio: 1:2 to 1:5
      • Medium Renewal: Two to three times weekly (ATCC)
    • Cryopreservation:
      • Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO (ATCC)
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • 3D cell culture
    • Immunology
  • Cell Line Availability: The cell line can be obtained from the following sources.
    • American Type Culture Collection (ATCC): Product link
    • DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
Author: admin

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