- Cell Line: SUP-HD1
- Synonyms:
- Sup-HD1
- SUP-HD-1
- SUPHD1
- Culture Type: Cell line
- Keywords: Human Hodgkin Lymphoma Cell Line
- Description: Established from Pleural effusion of a 37-year-old male at second (refractory/terminal) relapse of Hodgkin lymphoma after both combined chemo and radiotherapy
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 37 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue: Blood/Lymphoid
- Disease: Hodgkin lymphoma
- Primary/Metastasis:-
- Collection site: Pleural effusion
- Year of origin: 1987
- Country of Origin:-
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: mostly round (some polygonal) cells growing singly or in small clumps
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Doubling time: ~40-80 hours
- Karyotype: Human highly rearranged hyperdiploid karyotype with 4% polyploidy
- Microsatellite instability: Instable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- XPO1 (E571K mutation, Oncogenic, Gain-of-function)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-001203?tab=mutations
- https://depmap.org/portal/cell_line/ACH-001203?tab=fusions
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: 80% McCoy’s 5A + 20% heat-inactivated FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time: 1.5 x 106 cells/ml
- Seeding density: 0.3 – 0.5 x 106 cells/ml
- Recommended split ratio: 1:3 to 1:4
- Medium Renewal:-
- Subculturing method: Dilution
- Cryopreservation:
- Freezing medium: Complete growth medium + 20 % (v/v) FBS + 10 % (v/v) DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 20 % (v/v) FBS + 10 % (v/v) DMSO (ATCC)
- Applications:
- 3D cell culture
- Immunology
- Cell Line Availability: The cell line can be obtained from the following sources.
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- References
- Images:
- SUP-HD1 cell line image link 1 (Source: DSMZ)
- SUP-HD1 cell line image link 2 (Source: DSMZ)
- SUP-HD1 cell line image link 3 (Source: DSMZ)
- SUP-HD1 cell line image link 4(Source: DSMZ)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
