- Cell Line: SUP-M2
- Synonyms:
- Sup-M2
- SUP-M2
- SUPM2
- Culture Type: Cell line
- Keywords: Human Anaplastic Large Cell Lymphoma Cell Line
- Description: Established from Cerebrospinal fluid of a 5-year-old girl with refractory malignant histiocytosis
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 5 years
- Ethnicity: Caucasian
- Gender: Female
- Tissue: Lymphoid (DepMap)
- Disease: Anaplastic large cell lymphoma, ALK-positive
- Primary/Metastasis:-
- Collection site: Cerebrospinal fluid
- Year of origin:-
- Country of Origin:-
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Round cells growing singly or in clumps
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Doubling time: ~40-50 hours
- Karyotype: Human flat-moded near-diploid karyotype with 3.5% polyploid
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000226?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000226?tab=fusions
- For information, please visit
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI 1640 + 20% heat-inactivated FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time: 1.5 x 106 cells/ml
- Seeding density: 0.3 – 0.5 x 106 cells/ml
- Recommended split ratio: 1:3 to 1:4
- Medium Renewal:-
- Subculturing method: Dilution
- Cryopreservation:
- Freezing medium: Complete growth medium + 20 % (v/v) FBS + 10 % (v/v) DMSO (DSMZ)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): 6 x 106 cells/ml
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 20 % (v/v) FBS + 10 % (v/v) DMSO (DSMZ)
- Applications:
- Immunology
- Cell Line Availability: The cell line can be obtained from the following sources.
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- References
- Images:
- SUP-M2 cell line image link 1 (Source: DSMZ)
- SUP-M2 cell line image link 2 (Source: DSMZ)
- SUP-M2 cell line image link 3 (Source: DSMZ)
- SUP-M2 cell line image link 4 (Source: DSMZ)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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