- Cell Line: SW-1353
- Synonyms:
- SW-1353
- SW 1353
- SW1353
- Culture Type: Cell line
- Keywords: Human Chondrosarcoma Cell Line
- Description: Established from the bone of a 72-year-old, Caucasian female with chondrosarcoma
- Origin:
- Cell line features:
- Cell Type:-
- Morphology: Fibroblast-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: Hyperdiploid
- Doubling time:-
- Microsatellite instability:-
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- TP53 (V203L mutation, Likely Oncogenic, Likely Loss-of-function, Heterozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000418?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000418?tab=fusions
- TP53 (V203L mutation, Likely Oncogenic, Likely Loss-of-function, Heterozygous)
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Leibovitz’s L-15 Medium + 10% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 100% Air
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.03 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:2
- Medium Renewal: 2 to 3 times per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Applications:
- 3D cell culture
- Cancer research
- Transfection host
- Availability:
- American Type Culture Collection (ATCC):Product link
- Cytion: Product link
- References
- Images:
- SW-1353 cell line image link 1 (Source: Cytion)
- SW-1353 cell line image link 2 (Source: Cytion)
- SW-1353 cell line image link 3 (Source: Cytion)
- SW-1353 cell line image link 4 (Source: Cytion)
- SW-1353 cell line image link 5 (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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