- Cell Line: SW-403
- Synonyms:
- SW-403
- SW 403
- SW403
- Culture Type: Cell line
- Keywords: Human Colorectal Adenocarcinoma Cell Line
- Description: Established from a 51-year-old Caucasian female patient with Colorectal adenocarcinoma (Dukes’ type C, grade III)
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 51 years
- Ethnicity: Caucasian
- Gender: female
- Tissue: Large intestine, Colon
- Disease: Colorectal adenocarcinoma (Dukes’ type C, grade III)
- Primary/Metastasis: Primary (DepMap)
- Collection site: Colon (DepMap)
- Year of origin:-
- Country of Origin: United States (Culturecollections)
- Organism:
- Cell line features:
- Cell Type: Epithelial
- Morphology: Epithelial-like
- Growth mode: Adherent, Sometimes aggregated in complexes of 20-100 cells
- Life span: Infinite (continuous culture)
- Karyotype: modal number = 68, range = 59 to 73, Human hypotriploid karyotype with 12% polyploidy
- Doubling time: ~40 – 50 hours
- Microsatellite instability: Stable
- Special features: positive for keratin by immunoperoxidase staining
- Product: Carcinoembryonic antigen (CEA)
- Soft agar colony formation assay:-
- Tumorigenicity: tumorigenic in nude mice
- Gene expression:-
- Sequence variations:
- KRAS (G12V mutation, Oncogenic, Gain-of-function, Heterozygous)
- PIK3CA (Q546K, Oncogenic, Gain-of-function, Heterozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/SW403_LARGE_INTESTINE?tab=mutations
- https://depmap.org/portal/cell_line/SW403_LARGE_INTESTINE?tab=fusions
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC, DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- Leibovitz’s L-15 Medium + 10% FBS (ATCC)
- Leibovitz’s L-15 Medium + 10% FBS (ATCC)
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 100% Air
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time: Subconfluent culture (70% confluency) (Cells do not reach confluency but will grow in small islands)
- Seeding density:-
- Recommended split ratio: 1:2 to 1:6
- Medium Renewal: 2 to 3 times per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% (v/v) DMSO
- Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO (DSMZ)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- 3D cell culture
- Cancer research
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- Cytion: Product link Product link
- Sigma Aldrich: Product link
- References
- Images:
- SW-403 cell line image link 1 (Source: DSMZ)
- SW-403 cell line image link 2 (Source: DSMZ)
- SW-403 cell line image link 3 (Source: Cytion)
- SW-403 cell line image link 4 (Source: Cytion)
- SW-403 cell line image link 5 (Source: Cytion)
- SW-403 cell line image link 6 (Source: DepMap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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