- Cell Line: SW-480
- Synonyms:
- SW-480
- SW 480
- SW480
- SW480E
- Culture Type: Cell line
- Keywords: Human Colon Adenocarcinoma Cell Line
- Description: Established a 50-year-old Caucasian male patient with colon adenocarcinoma (grade 4, Duke class B)
- Origin:
- Cell line features:
- Cell Type: Epithelial
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: Human hypotriploid karyotype with 12% polyploidy
- Doubling time: 25-30 hours (DSMZ)
- Microsatellite instability: stable
- Special features: positive for keratin by immunoperoxidase staining
- Product: Carcinoembryonic antigen (CEA)
- Soft agar colony formation assay:-
- Tumorigenicity: tumorigenic in nude mice
- Gene expression:
- Sequence variations:
- TP53 (R273H (c.818G>A) mutation, Oncogenic, Loss-of-function, Heterozygous)
- KRAS (G12V mutation, Oncogenic, Gain-of-function, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000842?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000842?tab=fusions
- TP53 (R273H (c.818G>A) mutation, Oncogenic, Loss-of-function, Heterozygous)
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC, DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Leibovitz’s L-15 Medium + 10% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 100% Air
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:2 to 1:8
- Medium Renewal: 1 to 2 times per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Applications:
- 3D cell culture
- Cancer research
- Transfection host
- Availability:
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- Cytion: Product link
- SigmaAldrich: Product link
- References
- Images:
- SW-480 cell line image link 1 (Source: ATCC)
- SW 480 cell line image link 2 (Source: Culturecollections)
- SW 480 cell line image link 3 (Source: Culturecollections)
- SW 480 cell line image link 4 (Source: Culturecollections)
- SW 480 cell line image link 5 (Source: Cytion)
- SW 480 cell line image link 6 (Source: Cytion)
- SW 480 cell line image link 7 (Source: Cytion)
- SW 480 cell line image link 8 (Source: Cytion)
- SW 480 cell line image link 9 (Source: DSMZ)
- SW 480 cell line image link 10 (Source: DSMZ)
- SW 480 cell line image link 11 (Source: DSMZ)
- SW 480 cell line image link 12 (Source: DSMZ)
- SW 480 cell line image link 13 (Source: DSMZ)
- SW 480 cell line image link 14 (Source: DepMap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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