- Cell Line: SW-948
- Synonyms:
- SW-948
- SW 948
- SW948
- Culture Type: Cell line
- Keywords: Human Colon Adenocarcinoma Cell Line
- Description: Established a 81-year-old Caucasian female patient with colon adenocarcinoma (Dukes’ type C, grade III)
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 81 years
- Ethnicity: Caucasian
- Gender: female
- Tissue: Large intestine, Colon
- Disease: Colon adenocarcinoma (Dukes’ type C, grade III)
- Primary/Metastasis: Primary (DepMap)
- Collection site: Colon (DepMap)
- Year of origin:-
- Country of Origin: United States (Culturecollections)
- Organism:
- Cell line features:
- Cell Type: Epithelial
- Morphology: Epithelial-like
- Growth mode: Adherent, monolayer, form tight clusters or islands of epithelium-like cells
- Life span: Infinite (continuous culture)
- Karyotype: Human hypotriploid karyotype with 12% polyploidy (DSMZ)
- Doubling time: 2-4 days (Cellosaurus)
- Microsatellite instability: Stable
- Special features: positive for keratin by immunoperoxidase staining
- Product: Carcinoembryonic antigen (CEA)
- Soft agar colony formation assay:-
- Tumorigenicity: tumorigenic in nude mice
- Gene expression: positive for expression of c-myc, K-ras, H-ras, N-ras, myb and fos oncogenes
- Sequence variations:
- KRAS (Q61L mutation, Oncogenic, Gain-of-function, Heterozygous)
- PIK3CA (E542K mutation, Oncogenic, Gain-of-function)
- RHOA (G17E mutation, Likely Oncogenic, Likely Loss-of-function)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000680?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000680?tab=fusions
- KRAS (Q61L mutation, Oncogenic, Gain-of-function, Heterozygous)
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC, DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Leibovitz’s L-15 Medium + 10% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 100% Air
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.02 mM EDTA solution
- Harvesting time: Subconfluent culture (70% confluency)
- Seeding density:-
- Recommended split ratio: 1:2 to 1:5
- Medium Renewal: 1 to 2 times per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% (v/v) DMSO??????
- Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO (DSMZ)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- 3D cell culture
- Cancer research
- Transfection host
- Availability:
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- Cytion: Product link
- Sigma Aldrich: Product link
- References
- Images:
- SW-948 cell line image link 1 (Source: Culturecollections)
- SW-948 cell line image link 2 (Source: Cytion)
- SW-948 cell line image link 3 (Source: Cytion)
- SW-948 cell line image link 4 (Source: Cytion)
- SW-948 cell line image link 5 (Source: Cytion)
- SW-948 cell line image link 6 (Source: DSMZ)
- SW-948 cell line image link 7 (Source: DSMZ)
- SW-948 cell line image link 8 (Source: DSMZ)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
