Tag: plasmid
Plasmid: lp25
Plasmid name lp25 Host Borrelia burgdorferi Plasmid size(kbp) 24.2 Plasmid geometry Linear Plasmid copy number 1–2
Plasmid: ColE1
Plasmid name ColE1 Host Escherichia coli Plasmid size(kbp) 6.6 Plasmid geometry Circular Plasmid copy number 10–30
Plasmid: pUB110
Plasmid name pUB110 Host Bacillus subtilis Plasmid size(kbp) 2.3 Plasmid geometry Circular Plasmid copy number 20–50
Plasmid: Timeline of Key Events
1903: The chromosome theory of inheritance was developed independently by two researchers: Walter S. Sutton and Theodor Boveri 1910: The…
Plasmid vs Virus
Criteria Plasmid Virus Remarks Definition Small, circular or linear extrachromosomal DNA molecules found in bacteria and some eukaryotes Infectious acellular…
Plasmid vs Episome
Properties Plasmid Episome Remarks Molecular nature DNA DNA Both of them are made up of deoxyribonucleic acid Genetic material type…
Linear Plasmid
Linear plasmids represent a unique category of extrachromosomal genetic elements that differ from the more common circular plasmids in both…
Plasmid
Plasmids are extrachromosomal, double-stranded DNA molecules that are typically covalently closed and circular in structure.
List of Naturally Occurring Plasmids in Escherichia coli
pEC156 ColE1 pCoo REFERENCES Werbowy & Kaczorowski, 2016. Plasmid pEC156, a Naturally Occurring Escherichia coli Genetic Element That Carries Genes…
Plasmid-Encoded Traits
Resistance to antibiotics Production of antibiotics Degradation of complex organic compounds Production of bacteriocins Production of enterotoxins Restriction and modification…
Protocol: Double Digestion of Plasmid DNA with FastDigest EcoRV and HindIII Restriction Enzymes
A 100 – 200 ng plasmid DNA is sufficient to analyze by restriction digestion. Here we describe a procedure to double digest plasmid DNA with FastDigest EcoRV and HindIII restriction enzymes (from ThermoFisher Scientific). This procedure can be used to check the presence of a DNA fragment cloned in a vector. Double digestion will release the fragment of the appropriate size.
Restriction enzyme-free Cloning
In contrast to conventional cloning strategies, Restriction enzyme-free cloning (also called Restriction-free cloning) involves no treatment of DNA fragments to…
Protocol: Preparing T-vector using Terminal Transferase and ddTTP
T-vectors are linearized vectors that contain a single T-overhang at the 3’-end of both DNA strands. T-vectors are useful for…