Category: Lab Notes: Biotechniques
Ion-Pair Reversed-Phase High-Performance Liquid Chromatography (IP-RP-HPLC)
Ion-pair reversed-phase high-performance liquid chromatography (IP-RP-HPLC) is a powerful analytical technique used to separate and analyze ionic or highly polar…
Differences in Gene Expression Between Subconfluent and Confluent Cells
The transition from a subconfluent to a confluent state in cell culture represents a significant shift in cellular physiology and…
Fetal Bovine Serum (FBS) Alternatives in Cell Culture
Fetal bovine serum (FBS) has historically been a foundational supplement in cell culture media due to its rich content of…
Human Platelet Lysate
Human platelet lysate (HPL) is a human-derived, serum-free supplement used primarily in cell culture as a replacement for fetal bovine…
Fetal Bovine Serum (FBS) Composition
Fetal Bovine Serum (FBS) is a highly complex biological fluid with a rich and diverse composition that makes it uniquely…
Circadian Gene Expression in Cultured Cells
Circadian gene expression in cultured cells represents a fundamental area of chronobiology research that provides insights into the molecular mechanisms…
Fetal Bovine Serum (FBS)
Fetal Bovine Serum (FBS) is a complex biological product derived from the blood of bovine fetuses collected during the processing…
FlowJo
FlowJo represents a powerful and widely-used software platform specifically designed for the analysis and visualization of flow cytometry data. Originally…
Impact of Amphotericin B on Mitochondria in Mammalian Cell Cultures
Amphotericin B is a potent antifungal agent used in mammalian cell culture to prevent contamination by fungi, especially yeasts and…
Amphotericin B in Cell Culture
Amphotericin B is an antifungal agent widely used in cell culture to prevent contamination by fungi, including both yeasts and…
Impact of Kanamycin on Mitochondria in Mammalian Cell Cultures
Kanamycin, a member of the aminoglycoside antibiotic family, is known primarily for its use in bacterial systems to select for…
Kanamycin in Cell Culture
Kanamycin is an aminoglycoside antibiotic that inhibits bacterial protein synthesis by binding to the 30S subunit of prokaryotic ribosomes, causing…
Impact of Gentamicin on Mitochondria in Mammalian Cell Cultures
Gentamicin, like other aminoglycoside antibiotics, primarily targets bacterial ribosomes, binding to the 30S subunit to disrupt protein synthesis and cause…
Gentamicin in Cell Culture
Gentamicin is a broad-spectrum aminoglycoside antibiotic widely used in cell culture to prevent or control bacterial contamination, particularly from Gram-negative…
Impact of Streptomycin on Mitochondria in Mammalian Cell Cultures
Streptomycin is a widely used antibiotic in cell culture, particularly valued for its effectiveness against Gram-negative bacteria. It works by…