Category: Lab Notes: Biotechniques
Streptomycin in Cell Culture
Streptomycin is an aminoglycoside antibiotic widely used in cell culture to prevent bacterial contamination, especially from Gram-negative organisms. It is…
Impact of Penicillin on Mitochondria in Mammalian Cell Cultures
Penicillin targets bacterial cell wall synthesis by inhibiting enzymes (penicillin-binding proteins, PBPs) involved in the production of peptidoglycan. Mitochondria, although…
Penicillin in Cell Culture
Penicillin is one of the most commonly used antibiotics in cell culture, primarily employed to prevent bacterial contamination during the…
Cell Culture Antibiotics
Antibiotics are frequently used in cell culture to prevent or control microbial contamination. They can be helpful during the early…
Cell Culture Contamination
Cell culture contamination is one of the most common and serious problems encountered in laboratories involved in biological research and…
Primary Culture
Primary culture is the initial stage of cell culture, involving the isolation and growth of cells directly derived from tissues…
Animal Cell Culture
Animal cell culture is the process of growing animal-derived cells outside their natural biological context under controlled laboratory conditions. This…
Simoa (Single Molecule Array)
The Simoa (Single Molecule Array) technique is a highly sensitive digital immunoassay technology that allows for the detection of extremely…
TOPtkLuciferase Assay for Wnt Signaling
The TOPtkLuciferase reporter assay is a specific type of luciferase reporter system designed to monitor activity of the Wnt/β-catenin signaling…
Luciferase Assay
The luciferase assay is a powerful and widely used technique in molecular and cellular biology for monitoring gene expression, promoter…
Biolayer Interferometry
Biolayer Interferometry (BLI) is a label-free, optical biosensing technique used to monitor biomolecular interactions in real time. It is widely…
Quartz Crystal Microbalance (QCM) Immunosensors
Quartz Crystal Microbalance (QCM) immunosensors are highly sensitive biosensors that combine the principles of immunoassays with acoustic sensing technology to…
Mass Spectrometry Immunoassays
Mass spectrometry immunoassays (MSIAs) are powerful analytical techniques that combine the high specificity of immunoaffinity capture with the precision and…
Electrochemical Immunoassay
Electrochemical immunoassays are a class of biosensing techniques that combine the specificity of immunoassays with the sensitivity and simplicity of…
Immunoelectrophoresis
Immunoelectrophoresis is a laboratory technique that combines the principles of electrophoresis and immunodiffusion to separate and identify proteins, particularly antigens,…