Category: Sequencing
Mechanism of Chain Termination in Sanger Sequencing
In Sanger sequencing, a mixture of normal deoxyribonucleotides (dNTPs) and modified dideoxyribonucleotides (ddNTPs) is used to control DNA chain elongation. …
Sanger Sequencing: Historical Foundations to Modern Refinements
Sanger Sequencing, also known as the chain-termination method, stands as one of the most significant breakthroughs in molecular biology. Developed…
Sanger Sequencing Vs Next-Generation Sequencing
Criteria Sanger Sequencing Next-Generation Sequencing (NGS) Remarks Definition A first-generation DNA sequencing method based on selective incorporation of chain-terminating dideoxynucleotides…
High-Throughput Genomic Sequencing Technology
High-throughput genomic sequencing technologies, often referred to as next-generation sequencing (NGS), represent a transformative advancement in molecular biology and medicine,…
Sequencing
Sequencing refers to the experimental process of determining the precise order of the building blocks that make up a biological…
Whole-Genome Sequencing
Whole-Genome Sequencing (WGS) is a comprehensive method used to determine the complete DNA sequence of an organism’s genome. This process…
Ion Torrent Sequencing
Ion Torrent sequencing is also known as Ion semiconductor sequencing. For more detailed information, please visit the article Ion Semiconductor…
Ion Semiconductor Sequencing
Ion semiconductor sequencing, also known as Ion Torrent sequencing, represents a significant advancement in next-generation sequencing technology. This method utilizes…
Alanine tRNA and the Birth of Sequencing: The Legacy of Robert W. Holley
In the annals of molecular biology, the sequencing of alanine transfer RNA (tRNA) from yeast stands as a monumental achievement….
Automation of Sanger Sequencing Using Fluorescent Dideoxynucleotides and Capillary Electrophoresis
Sanger sequencing, developed by Frederick Sanger in the late 1970s, was the first widely adopted method for determining the nucleotide…
DNA Sequencer
A DNA sequencer is a laboratory instrument used to determine the precise order of nucleotides—adenine (A), thymine (T), cytosine (C),…
DNA Sequencing: Timeline of Key Events
DNA sequencing, the process of determining the precise order of nucleotides in a DNA molecule, has revolutionized biology, biotechnology, and…
Shotgun Sequencing
Shotgun sequencing is a powerful and widely used approach to DNA sequencing that involves randomly breaking a genome into numerous…
Maxam–Gilbert Sequencing
Maxam–Gilbert sequencing, also known as chemical sequencing, is one of the first DNA sequencing methods ever developed. Introduced in 1977…
First-Generation Sequencing
First-Generation Sequencing encompasses the earliest set of DNA sequencing technologies developed in the 1970s and 1980s, which provided the first…