Overview | |
| Organism | Human (Homo sapiens), 6 years old, Male, White (Friedman et al., 1985) |
| Source/Tissue | Brain, Cerebellum, Medulloblastoma REF |
| Morphology | Epithelial REF |
| Cell type | |
| Growth mode | multicellular aggregates, some adherent REF |
| Doubling time | 52.55 h (Friedman et al., 1985) |
| Life span | Infinite, continuous culture (Friedman et al., 1985) |
| Karyotype | Hypodiploid REF |
| Transformed | Naturally transformed (anchorage independent growth) |
| Tumorigenic | Tumorigenic in nude mice (Friedman et al., 1985) |
| Drug resistance | |
| Biosafety Level | 1 REF (May differ in your country, follow the guidelines of your institute/country) |
| Gene Expression | |
| Products | |
| Applications | |
| Remarks | |
| Related cell lines |
Cell line isolation information
The cell line D283 Med was established from malignant ascites cells and a peritoneal metastasis from a 6 years old boy with medulloblastoma. (Friedman et al., 1985)
Recommended propagation method
Culture medium:
Eagle’s Minimum Essential Medium (+ 2mM Glutamine) + 10% FBS REF
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C REF
Split ratio:
Split frequency:
Harvesting time: Subconfluent culture (≈ 80% )
Split Method: Dilution REF
Recommended Cryopreservation Method
Freeze medium: 70% complete culture medium + 20% FBS + 10% DMSO
Storage temperature: Liquid nitrogen vapor phase / -150 °C freezer
Cell density: ?
Availability
- American Type Culture Collection (ATCC) : Product Link
REFERENCES
- Friedman et al., 1985. Establishment and characterization of the human medulloblastoma cell line and transplantable xenograft D283 Med. J Neuropathol Exp Neurol. 44(6), 592-605. PMID-4056828; Full Text Link: academic.oup
- American Type Culture Collection (ATCC)
