- Cell Line: HCC1500
- Synonyms:
- HCC-1500
- Hamon Cancer Center 1500
- Culture Type: Cell line
- Keywords: Human epithelial mammary ductal carcinoma cell line
- Description: Established from the mammary gland of a 32-year-old, Black female patient with breast invasive ductal carcinoma TMN stage IIB, grade 2.
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 32 years
- Ethnicity: Black
- Gender: Female
- Tissue (origin): Breast, Mammary gland
- Tissue (isolation): Breast, Mammary gland
- Disease:
- Breast Invasive Ductal Carcinoma TNM Stage IIB, grade 2
- Primary/Metastasis: Primary
- Molecular subtype: ERpos HER2neg (depmap)
- Breast Invasive Ductal Carcinoma TNM Stage IIB, grade 2
- Year of origin: 1995
- Organism:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial cell
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Clinically relevant genes:
- Gene expression:
- Positive for the expression of Epithelial glycoprotein 2 (EGP2) (epithelial cell-specific marker)
- Positive for the expression of Cytokeratin 19 (epithelial cell-specific marker)
- Sequence variations (gene mutations/deletions/fusions/translocations):
- Morphology: Epithelial-like
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:
- Split ratio: 1:2 to 1:3
- Medium Renewal: Every 3 to 5 days
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- https://www.atcc.org/products/crl-2329
- https://www.cellosaurus.org/CVCL_1254
- https://depmap.org/portal/cell_line/ACH-000349?tab=overview
- Gazdar et al., 1998. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PMID-9833771; Full Text: Wiley (Download PDF)
- Images
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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