Description: HEK293-A cells (a subclone of HEK-293) stably expressing mRFP-tagged human ATG9
- Synonyms: HEK293-A mRFP-ATG9
- Keywords: HEK293-A cells (a subclone of HEK-293) stably expressing mRFP-tagged human ATG9
- Culture Type: Cell line
- Origin/Source:
- Organism:
- Species: Human (Homo sapiens)
- Age: Fetus
- Tissue of Origin: Embryonic Kidney
- Organism:
- Cell line features:
- Morphology: Neuronal
- Cell Type: Neuronal
- Growth Mode: Adherent
- Morphology: Neuronal
- Applications:
- Autophagy analysis
- Handling and maintenance
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- GMO Status: GMO1
- Complete culture medium:
- DMEM + 2mM Glutamine + 10% FBS + G418 (400 µg/ml)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 10% CO₂, 90% Air (culturecollections)
- Subculturing:
- Cell dissociation procedure: 0.05% Trypsin/EDTA or Trypsin
- Harvesting time: 80% confluence culture
- Subcultivation Ratio:
- Medium Renewal:
- Seeding densities: 2×10⁴ cells/cm²
- Cryopreservation
- Growth medium + 10% DMSO
- Cryopreservation temperature: Liquid nitrogen gas phase or -150°C freezer
- Availability:
- Culture Collections (UK Health Security Agency): Catalogue No. Product link
- References:
- https://www.phe-culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=17100531&collection=ecacc_gc
- Orsi et al., 2012. Dynamic and transient interactions of Atg9 with autophagosomes, but not membrane integration, are required for autophagy. Mol Biol Cell. 23(10), 1860-73. PMID: 22456507; Full-Text Links: Molbiolcell, PMC
- Young et al., 2006. Starvation and ULK1-dependent cycling of mammalian Atg9 between the TGN and endosomes. J Cell Sci. 119(Pt 18), 3888-900. PMID: 16940348; Full-Text Links: Biologists, ilverchair-cdn
- Abbreviations
- DMEM (Dulbecco’s Modified Eagle’s Medium)
- FBS (Foetal Bovine Serum)
- GMO1 (Genetically Modified Organism Class 1)
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