| OVERVIEW | |
| Organism | Human (Homo sapiens), 15 years old white male (Ref: ATCC) |
| Source/Tissue of origin | Liver (Ref: ATCC) Established from hepatocellular carcinoma (Ref: ATCC) |
| Year of isolation | 1975 (Ref: ATCC) |
| Morphology | Epithelial-like (Ref: ATCC) |
| Cell type | Epithelial (Ref: ATCC) |
| Growth mode | Adherent (Ref: ATCC) |
| Doubling time | ? |
| Life span | Infinite, continuous culture (Ref: ATCC) |
| Karyotype | Modal chromosome number = 55 (Ref: ATCC) |
| Transformed | Naturally transformed (can form colonies in semisolid medium) (Ref: ATCC) |
| Tumorigenic | Yes (in nude mice) (Ref: ATCC) |
| Drug resistance | ? |
| Gene Expression | |
| Positive for | Albumin (Ref: ATCC) |
| Altered gene | |
| Enzymes | |
| Products | |
| Applications | 3D cell culture (Ref: ATCC) Suitable transfection host (Ref: ATCC) Cancer research (Ref: ATCC) High-throughput screening (Ref: ATCC) |
| Remarks | |
| Keywords | Human liver cancer cell line |
| Related cell lines | |
| Culture Maintenance | |
| Biosafety Level | BSL 1 (Ref: ATCC) (It may differ in your country, follow the guidelines of your institute/country) |
| Culture medium | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% FBS (Ref: ATCC) |
| Culture conditions | Humidified incubator, 37°C, 5% CO2 (Ref: ATCC) |
| Subculturing procedure | Trypsin-EDTA |
| Culture storage | |
| Cryo-medium | Complete growth medium + 5% (v/v) (Ref: ATCC) |
| Storage conditions | Vapor phase of liquid nitrogen |
