- Cell Line: NCI-H1048
- Synonyms:
- NCI-H1048
- NCIH1048
- H1048
- H-1048
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Carcinoma Epithelial Cell Line
- Description: Established from the pleural effusion (metastatic site) of a 53-year-old, African American female patient with Small Cell Lung Carcinoma (Neuroendocrine Tumor)
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 53 years
- Ethnicity: African American
- Gender: Female
- Tissue (origin): Lung
- Tissue (isolation): Pleural effusion (metastatic site)
- Disease:
- Small Cell Lung Carcinoma/Lung Neuroendocrine Tumor
- Primary/Metastasis: Metastatic
- Small Cell Lung Carcinoma/Lung Neuroendocrine Tumor
- Year of origin: 1985
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Microsatellite instability: Instable
- Special features:-
- Product:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- TP53 (R273C mutation, Likely Oncogenic, Likely Loss-of-function, Heterozygous)
- PIK3CA (H1047R mutation, Oncogenic Gain-of-function, Heterozygous)
- PIK3CA (K111R mutation, Likely Oncogenic, Likely Gain-of-function)
- RRAS2 (G23S mutation, Likely Oncogenic, Likely Gain-of-function)
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- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM:F12 Medium (Catalog No.30-2006) + 0.005 mg/ml Insulin + 0.01 mg/ml Transferrin + 30nM Sodium selenite (final conc.) + 10 nM Hydrocortisone (final conc.) + 10 nM beta-estradiol (final conc.) + extra 2mM L-glutamine (for final conc. of 4.5 mM) + 5% fetal bovine serum (final conc.)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:3 to 1:8
- Medium Renewal:-
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- AddexBio Technologies: Product link
- References
- Images:
- NCI-H1048 cell image link (Source: ATCC)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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