- Cell Line: NCI-H1395
- Synonyms:
- NCIH1395
- NCI-H1395
- H1395
- H-1395
- Culture Type: Cell line
- Keywords: Human Lung Adenocarcinoma Epithelial Cell Line
- Description: Established from tumor tissue of a 55-year-old, White female with Non-Small Cell Lung adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 55 years
- Ethnicity: White
- Gender: Female
- Tissue (origin): Lung
- Tissue (isolation): Lung (tumor tissue)
- Disease:
- Adenocarcinoma
- Molecular subtype: Non-Small Cell Lung adenocarcinoma
- Molecular subtype: Non-Small Cell Lung adenocarcinoma
- Primary/Metastasis: Primary
- Adenocarcinoma
- Year of origin: 1986
- Organism:
- Cell line features:
- Cell Type: Epithelial cell
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time: ≈ 50.5 h
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- ATM (T2666A mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- BRAF (G469A mutation, Oncogenic, Gain-of-function)
- PTPRT (Q479E mutation, Likely Oncogenic, Likely Loss-of-function)
- For more information, please visit
- https://depmap.org/portal/cell_line/ACH-000327?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000327?tab=fusions
- https://depmap.org/portal/cell_line/ACH-000327?tab=translocations
- Cell Type: Epithelial cell
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:3 to 1:6
- Medium Renewal:-
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- Cyton: Product number: Product link
- References
- Images:-
- NCI-H1395 cell image 1 (Source: Cyton)
- NCI-H1395 cell image 2 (Source: Cyton)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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