- Cell Line: NCI-H1581
- Synonyms:
- NCI-H1581
- NCIH1581
- H1581
- H-1581
- Culture Type: Cell line
- Keywords: Human Lung Carcinoma Cell Line
- Description: Established from the lungs of a 44-year-old, Caucasian male patient with stage 4 non-small cell lung carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 44 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Disease:
- Non-Small Cell Lung Carcinama, Stage 4
- Molecular subtype NSCLC Large Cell
- Molecular subtype NSCLC Large Cell
- Primary/Metastasis: Primary
- Non-Small Cell Lung Carcinama, Stage 4
- Year of origin: 1987
- Organism:
- Cell line features:
- Cell Type: Large cell
- Morphology: Round cell clusters
- Growth mode: Mixed: adherent and suspension
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- Cell Type: Large cell
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- ACL4 [DMEM:F12 + 1X ACL-4 Cocktail] + 5 mg/mL Bovine Serum Albumin + 0.01 mg/mL Transferrin + 1 ng/mL EGF (ATCC)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution (Addition or replacement of culture medium)
- Harvesting time:-
- Split ratio:-
- Medium Renewal:-
- Subculturing method: Dilution (Addition or replacement of culture medium)
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 10% (v/v) FBS + 5% (v/v) DMSO
- Complete growth medium + 10% (v/v) FBS + 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- Images:–
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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