- Cell Line: NCI-H1755
- Synonyms:
- NCI-H1755
- NCIH1755
- H1755
- H-1755
- Culture Type: Cell line
- Keywords: Human Lung Adenocarcinoma Cell line
- Description: Established from the metastatic tumor (Liver) of a 65-year-old caucasian female with non-small cell lung cancer
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 65 years
- Ethnicity: Caucasian
- Gender: Female
- Smoking habit: smoker
- Tissue (origin): Lung
- Disease: Non-small cell lung cancer
- Disease subtype: Lung Adenocarcinoma
- Molecular subtype:-
- Primary/Metastasis: Metastatic (Liver)
- Year of origin: 1987
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like and/or rounded
- Growth mode: Mixed: Adherent and small clusters in suspension
- Life span: Infinite (continuous culture)
- Karyotype:-
Doubling time: – - Microsatellite instability: Stable
- Special features:
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:-
- Cell Type:-
- Handling and maintenance:-
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution (suspension cells) and Enzymatic dissociation (attached cells) using 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Cell dissociation solution:
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:3 to 1:10
- Medium Renewal: Every 3 to 4 days
- Subculturing method: Dilution (suspension cells) and Enzymatic dissociation (attached cells) using 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 40% (v/v) FBS + 5% (v/v) DMSO
- Complete growth medium + 40% (v/v) FBS + 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
