- Cell Line: NCI-H596
- Synonyms:
- NCIH596
- NCI-H596
- H596
- H-596
- Culture Type: Cell line
- Keywords: Human Lung Adenosquamous Carcinoma Epithelial Cell Line
- Description: Established from the primary tumor tissue of a 73-year-old, White, male with lung Adenosquamous Carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 73 years
- Ethnicity: White
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Lung (tumor tissue)
- Disease:
- Non-Small Cell Lung Cancer
- Molecular subtype: NSCLC Adenosquamous
- Molecular subtype: NSCLC Adenosquamous
- Primary/Metastasis: Primary
- Non-Small Cell Lung Cancer
- Year of origin: 1983
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: Modal number = 71, range = 65 to 75
- Doubling time: ≈ 41.1 h
- Microsatellite instability: Stable
- Special features:-
- Product: Involucrin
- Tumorigenicity: Yes, in nude mice
- Gene expression:
- p53 mRNA positive
- positive for keratin
- positive for vimentin
- negative for neurofilament triplet protein
- Sequence variations:
- PIK3CA (E545K mutation, Oncogenic, Gain-of-function, Heterozygous)
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- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:4 to 1:8
- Medium Renewal: 2 to 3 times per week
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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