- Cell Line: SK-BR-3
- Synonyms:
- SK-BR-3
- Sk-BR-3
- Sk-Br-3
- SK-BR3
- SKBR-3
- SKBr-3
- SkBr-3
- SK BR 03
- SKBR3
- Culture Type: Cell line
- Keywords: Human Breast Adenocarcinoma Cell Line
- Description: Established from pleural effusion tumor cells of a 43-year-old Caucasian female patient with breast adenocarcinoma. The patient had been treated with radiation, steroids, cytoxan, and 5-fluorouracil.
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Ethnicity: Caucasian
- Age: 43 years
- Gender: Female
- Species: Human (Homo sapiens)
- Tissue: Breast, Mammary gland
- Disease: Breast adenocarcinoma
- Primary/Metastasis: Metastatic
- Collection site: Pleural effusion
- Year of origin: 1970
- Country of Origin: United States
- Organism:
- Cell line features:
- Cell Type: Epithelium?
- Morphology: Epithelial-like
- Growth mode: Adherent, monolayer
- Life span: Infinite (continuous culture)
- Doubling time:
- ~ 37.4 hours (Cellosaurus)
- ~30 hours (Cellosaurus)
- Karyotype: Hypertriploid cell line, Modal chromosome number: 84
- Microsatellite instability: Stable (MSS)
- Special features: Overexpresses the HER2/c-erb-2 gene product
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity: Yes, in nude mice
- Gene expression:
- Sequence variations:
- TP53 (R175H mutation, Oncogenic, Loss-of-function, Homozygous)
- For more information, please visit:
- https://depmap.org/portal/cell_line/SKBR3_BREAST?tab=mutations
- https://depmap.org/portal/cell_line/SKBR3_BREAST?tab=fusions
- Cell Type: Epithelium?
- Handling and maintenance:
- Biosafety level: BSL 1 (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: McCoy’s 5a Medium + 10% FBS (ATCC)
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% trypsin/0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Split ratio: 1:2
- Split frequency:-
- Medium Renewal: 2 to 3 times per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% (v/v) DMSO
- Applications:
- Cancer research
- 3D cell culture
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- BCRJ: Product link
- Cytion: Product link
- References
- Images:
- SK-BR-3 cell line image link 1 (Source: ATCC)
- SK-BR-3 cell line image link 2 (Source: Cytion)
- SK-BR-3 cell line image link 3 (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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