- Cell Line: SK-LU-1
- Synonyms:
- SK-LU-1
- SK-LU1
- SK LU 1
- SKLU1
- SK-Lu1
- SK-Lu-1
- Culture Type: Cell line
- Keywords: Human Lung Adenocarcinoma Cell Line
- Description: Established from the lungs of a 60-year-old, Caucasian female patient with adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age:
- 60 years (ATCC)
- 64 years (Culturecollections)
- Ethnicity: Caucasian
- Gender: Female
- Tissue: Lung
- Disease: Adenocarcinoma
- Primary/Metastasis: Primary (DepMap)
- Collection site: Lung (DepMap)
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: Epithelial
- Morphology: Epithelial-like
- Growth mode: Adherent, monolayer
- Life span: Infinite (continuous culture)
- Karyotype: Hypotetraploid
- Doubling time:-
- Microsatellite instability: stable
- Special features:-
- Product:
- Soft agar colony formation assay:-
- Tumorigenicity: tumorigenic in immunotolerant rats
- Gene expression:
- Sequence variations:
- TP53 (H193R mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- KRAS (G12D mutation, Oncogenic, Gain-of-function, Heterozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000309?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000309?tab=fusions
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Eagle’s Minimum Essential Medium + 10% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:2
- Medium Renewal: Twice per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium: Culture medium + 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Culture medium + 5% (v/v) DMSO
- Applications:
- 3D cell culture
- Cancer research
- Availability:
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- SigmaAldrich : Product link
- Cytion: Product link
- References
- Images:
- SK-LU-1 cell line image link 1 (Source: Culturecollections)
- SK-LU-1 cell line image link 2 (Source: Culturecollections)
- SK-LU-1 cell line image link 3 (Source: Culturecollections)
- SK-LU-1 cell line image link 4 (Source: Cytion)
- SK-LU-1 cell line image link 5 (Source: Cytion)
- SK-LU-1 cell line image link 6 (Source: Cytion)
- SK-LU-1 cell line image link 7 (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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