- Cell Line: SK-MEL-1
- Synonyms:
- SK-MEL-1
- SKMEL1
- SkMEL-1
- SK-Mel-1
- SK Mel 1
- SK-Mel1
- Culture Type: Cell line
- Keywords: Human Melanoma Cell Line
- Description: Established from the lymph node metastasis (thoracic duct) of a 29-year-old Caucasian male with primary melanoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 29 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue: Skin
- Disease: Melanoma
- Primary/Metastasis: Metastatic (DepMap)
- Collection site: Thoracic lymph duct
- Year of origin: 1966
- Country of Origin:-
- Organism:
- Cell line features:
- Cell Type:
- Morphology: Mostly round cells growing singly or in clumps
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Doubling time: ~100 hours (DSMZ)
- Karyotype: Human hypertriploid karyotype with 4% polyploid
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:
- Yes, in nude mice
- pigment-producing tumors upon heterotransplantation into hamster (DSMZ)
- Gene expression:-
- Sequence variations:
- CTNNB1 (S33C mutation, Oncogenic, Gain-of-function, Heterozygous)
- BRAF (V600E mutation, Oncogenic, Gain-of-function, Heterozygous)
- For more information, please visit
- https://depmap.org/portal/cell_line/ACH-000465?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000465?tab=fusions
- Handling and maintenance:
- Biosafety level: BSL 1 (DSMZ, ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time: 0.5-1.0 x 106 cells/ml
- Seeding density: 1 x 105 cells/mL
- Split ratio:-
- Split frequency:-
- Medium Renewal: 2 to 3 times per week
- Subculturing method: Dilution
- Cryopreservation:
- Applications:
- 3D cell culture
- Cancer research
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- Cytion: Product number: Product link
- References
- Images:
- SK-MEL-1 cell line image link 1 (Source: ATCC)
- SK-MEL-1 cell line image link 2 (Source: DSMZ)
- SK-MEL-1 cell line image link 3 (Source: DSMZ)
- SK-MEL-1 cell line image link 4 (Source: Cytion)
- SK-MEL-1 cell line image link 5 (Source: Cytion)
- SK-MEL-1 cell line image link 6 (Source: Cytion)
- SK-MEL-1 cell line image link 7 (Source: Cytion)
- SK-MEL-1 cell line image link 8 (Source: DepMap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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