- Cell Line: SU-DHL-10
- Synonyms:
- SU-DHL-10
- SUDHL10
- SUDHL-10
- SuDHL-10
- SuDHL 10
- Stanford University-Diffuse Histiocytic Lymphoma-10
- Culture Type: Cell line
- Keywords: Human B Cell Lymphoma Cell Line
- Description: Established from the lymph node of a 25- year-old Caucasian male patient with Large Cell Lymphoma
- Origin:
- Cell line features:
- Cell Type: B lymphocyte
- Morphology: Lymphoblast-like, round lymphoid cells growing singly or in clumps
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype: hyperdiploid karyotype (DSMZ)
- Doubling time: ~ 15-30 hours (DSMZ)
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- PTEN (Y68H mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- PTEN (D162H mutation, Oncogenic, Loss-of-function)
- EP300 (L415P mutation, Likely Oncogenic, Unknown)
- CCND3 (I290T mutation, Likely Oncogenic, Likely Gain-of-function)
- EZH2 (Y646F mutation, Likely Oncogenic, Likely Gain-of-function)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000271?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000271?tab=fusions
- Cell Type: B lymphocyte
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC, DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- RPMI-1640 Medium + 10% FBS
- RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Cryopreservation:
- Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO (ATCC)
- Applications:
- 3D cell culture
- Immunology
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- References
- Images:
- SU-DHL-10 cell line image link 1 (Source: ATCC)
- SU-DHL-10 cell line image link 2 (Source: DSMZ)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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