- Cell Line: SUP-T1
- Synonyms:
- SUP-T1
- SUPT1
- SUPT-1
- SUP T-1
- Sup-T1
- Sup T-1
- SupT1
- Stanford University Pediatric T-cell line 1
- Culture Type: Cell line
- Keywords: Human T Cell Lymphoma Cell Line
- Description: Established from pleural effusion of an 8 year old Caucasian child with T-Cell Lymphoblastic Lymphoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 8 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue: Blood/Lymphoid
- Disease: T-Cell Lymphoblastic Lymphoma
- Primary/Metastasis: Metastatic (DepMap)
- Collection site: Pleural effusion
- Year of origin:
- Country of Origin: United States
- Organism:
- Cell line features:
- Cell Type: T lymphoblast
- Morphology: Lymphoblast-like; single, round cells
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Doubling time: ~ 30 hours (DSMZ)
- Karyotype: Human hypotetraploid karyotype with 1.8% polyploidy (DSMZ)
- Microsatellite instability:
- Stable (MSS) (Sanger). (Cellosaurus)
- Instable (MSI) (PubMed=31068700). (Cellosaurus)
- Special features: The cells express multiple T lineage markers
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- MPL (A497T mutation, Likely Oncogenic, Likely Gain-of-function)
- INPPL1 (R70H mutation, Likely Oncogenic, Unknown)
- EP300 (L415P mutation, Likely Oncogenic, Unknown)
- TP53 (R267L mutation, Likely Oncogenic, Likely Gain-of-function, Heterozygous)
- TP53 (R273H mutation, Oncogenic, Loss-of-function)
- TP53 (R248Q mutation, Likely Oncogenic, Likely Loss-of-function)
- PIK3CA (E970K mutation, Likely Oncogenic, Likely Gain-of-function)
- CARD11 (E134K mutation, Likely Oncogenic, Likely Gain-of-function)
- PIK3CA (E545D mutation, Likely Oncogenic, Likely Gain-of-function, Heterozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000953?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000953?tab=fusions
- Cell Type: T lymphoblast
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC, DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time: 2 x 106 cells/mL
- Seeding density: 3 x 105 viable cells/mL
- Recommended split ratio: 1:4 to 1:6
- Medium Renewal: 2 to 3 times per week
- Subculturing method: Dilution
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Applications:
- 3D cell culture
- Immunology
- Transfection host
- Useful for studies of cell fusion and cytopathic effects of HIV-1 as well as cytopathic isolates of HIV-2
- Susceptible to infection by herpesvirus 6B (HHV-6B) strain Z29
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- UK Health Security Agency Culture Collections: Product link
- SigmaAldrich: Product link
- References
- Images:
- SUP-T1 cell line image link 1 (Source: ATCC)
- SUP-T1 cell line image link 2 (Culturecollections)
- SUP-T1 cell line image link 3; (Source: DSMZ)
- SUP-T1 cell line image link 4 (Source: DSMZ)
- SUP-T1 cell line image link 5 (Source: DSMZ)
- SUP-T1 cell line image link 6 (Source: DSMZ)
- SUP-T1 cell line image link 7 (Source: DSMZ)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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