Dulbecco’s modified Eagle’s medium is extensively used for animal tissue culture.
| Composition of Dulbecco’s modified Eagle’s medium | |
| Ingredients | Concentration |
| Inorganic Salts | |
| Calcium Chloride (CaCl2) (anhydrous) | 200 mg/L |
| Ferric Nitrate (Fe(NO3)3.9H2O) | 0.1 mg/L |
| Magnesium Sulfate (MgSO4.7H2O) | 200 mg/L |
| Potassium Chloride (KCl) | 400 mg/L |
| Sodium Bicarbonate (NaHCO3) | 3700 mg/L |
| Sodium Chloride (NaCl) | 6400 mg/L |
| Sodium Phosphate monobasic (NaH2PO4.H2O) | 125 mg/L |
| Sodium pyruvate | 110 mg/L |
| Amino Acids | |
| L-Arginine.HCl | 84 mg/L |
| L-Cystine * | 48 mg/L |
| L-Histidine.HCl.H2O | 42 mg/L |
| L-Isoleucine | 105 mg/L |
| L-Glutamine | 580 mg/L |
| Glycine | 30 mg/L |
| L-Leucine | 105 mg/L |
| L-Lysine.H2O * | 150 mg/L |
| L-Methionine | 30 mg/L |
| L-Phenylalanine | 66 mg/L |
| L-Serine | 42 mg/L |
| L-Threonine | 95 mg/L |
| L-Tryptophan | 16 mg/L |
| L-Tyrosine | 72 mg/L |
| L-Valine | 94 mg/L |
| Vitamins | |
| D-Calcium pantothenate | 4 mg/L |
| Choline chloride | 4 mg/L |
| Folic acid | 4 mg/L |
| i-Inositol | 7.2 mg/L |
| Nicotinamide | 4 mg/L |
| Pyridoxal.HCl | 4 mg/L |
| Riboflavin | 0.4 mg/L |
| Thiamine HCl | 4 mg/L |
| Other components | |
| Glucose | 4500 mg/L |
| Phenol Red | 15 mg/L |
