| OVERVIEW | |
| Organism | Human (Homo sapiens), 36 years old white female |
| Year of isolation | |
| Source/Tissue of origin | Peripheral blood, Acute promyelocytic leukemia |
| Morphology | Lymphoblast-like |
| Cell type | Promyeloblast |
| Growth mode | Suspension |
| Doubling time | about 40 hours (dsmz) |
| Life span | Infinite, continuous culture |
| Karyotype | Pseudodiploid |
| Transformed | |
| Tumorigenic | Tumorigenic in nude mice |
| Drug resistance | |
| Biosafety Level | 1 (dsmz, atcc) (May differ in your country, follow the guidelines of your institute/country) |
| Gene Expression | TNF-alpha |
| Products | |
| Applications | |
| Remarks | Differentiate spontaneously. Differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid, dimethylsulfoxide, actinomycin D, and retinoic acid. |
| Related cell lines |
Cell line isolation information
The cell line HL-60 was established from the peripheral blood of a 35-year-old woman with acute myeloid leukemia (AML FAB M2) in 1976.
Recommended propagation method
Culture medium:
1. RPMI 1640 + 10% h.i. FBS (dsmz)
2. Iscove’s Modified Dulbecco’s Medium + 20% FBS (atcc)
Culture conditions: Humidified incubator, 95% air + 5% CO2, 37°C.
Split ratio: 1:2 to 1:5
Split frequency: every 2-3 days
Harvesting time: 1 X 106 (Do not allow cell concentration to exceed 1 X 106 (atcc))
Split Method: Dilution
Seeding density: 1 X 105/ml
Recommended Cryopreservation Method
Freeze medium:
1. complete medium + 20% FBS + 10% DMSO (dsmz)
2. Iscove’s Modified Dulbecco’s Medium + 7% FBS + 5% (v/v) DMSO (atcc)
Storage temperature: Liquid nitrogen vapor phase
Cell density: 1 X 106 cells /ml
AVAILABILITY
- Public Health England : Product link
- Cell Bank Australia: Product link
REFERENCES
- Gallagher et al., 1979. Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. Blood. 54(3), 713-733. PMID-288488; Full-text Link: sciencedirect (download PDF)
