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DNA damage

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HindIII Restriction Enzyme

 admin  October 14, 2021  0 Comments on HindIII Restriction Enzyme

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific sites Recognize hexameric palindromic sequence Sticky end cutter (cuts both…

Deoxyribose

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Molecular formula C5H10O4 Molecular weight 134.131 Molar mass  134.131g/mol IUPAC name  2-deoxy-d-ribose CAS Number  533-67-5 Chemically, Deoxyribose (or 2-deoxyribose) is…

Nitrogenous Bases

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Nitrogenous bases (also known as Nucleobases or bases) are nitrogen-containing compounds. They are an important component of nucleic acids. These…

EcoRI Restriction Enzyme

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Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific cleavage sites Recognize hexameric palindromic sequence Sticky end cutter (cuts…

PmeI Restriction Enzyme

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Synonyms: MssI Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific sites Recognize octameric palindromic sequence Blunt-end cutter (cut…

NheI Restriction Enzyme

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Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific sites Recognize hexameric palindromic sequence Sticky end cutter (cut both…

DNA Structure

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Ribose

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Chemically, Ribose is a single-ring 5-carbon (pentose) sugar. Ribose is an essential component of ribonucleic acid (RNA). It can exist…

ApaI Restriction Enzyme

 admin  October 12, 2021  0 Comments on ApaI Restriction Enzyme

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric inverted repeat palindromic sequence Sticky end…

SmaI Restriction Enzyme

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Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Blunt-end cutter (cut both…

NotI Restriction Enzyme

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Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize octameric palindromic sequence Sticky end cutter (cut…

Protocol: Preparing T-vector using Terminal Transferase and ddTTP

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T-vectors are linearized vectors that contain a single T-overhang at the 3’-end of both DNA strands. T-vectors are useful for…

XbaI Restriction Enzyme

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Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific sites Recognize hexameric palindromic sequence Sticky end cutter (cut both…

BamHI Restriction Enzyme

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Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Sticky end cutter (cut…