Category: Lab Notes: Biotechniques

Multiphoton Microscopy

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Multiphoton microscopy (MPM) is an advanced fluorescence imaging technique that enables high-resolution, three-dimensional visualization of biological specimens, particularly deep within…

Compound Microscope

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A compound microscope is a fundamental optical instrument used to magnify small objects that are invisible to the naked eye….

Polarizing Microscopy

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Polarizing microscopy, also known as polarized light microscopy (PLM), is a specialized optical microscopy technique used to observe and analyze…

Scanning Tunneling Microscope (STM)

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A Scanning Tunneling Microscope (STM) is a revolutionary instrument that achieves atomic-scale imaging by utilizing quantum tunneling effects between a…

Atomic Force Microscope (AFM)

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An Atomic Force Microscope (AFM) is a sophisticated scanning probe microscope that achieves atomic-scale resolution by measuring forces between a…

Structured Illumination Light Sheet Microscopy (SILSM)

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Structured Illumination Light Sheet Microscopy (SILSM) is an advanced optical imaging technique that combines the strengths of two powerful microscopy…

Structured Illumination Microscopy

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Structured illumination microscopy (SIM) is a powerful super-resolution imaging technique that enhances spatial resolution beyond the diffraction limit of conventional…

Microscopy

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Microscopy is a foundational technique in science that allows for the observation of structures too small to be seen with…

Light-Sheet Fluorescence Microscopy (LSFM)

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Light-sheet fluorescence microscopy (LSFM), also known as selective plane illumination microscopy (SPIM), is an advanced imaging technique that allows rapid,…

Optical Sectioning

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Optical sectioning is a microscopy technique that enables the capture of thin, focused slices (or “sections”) of a specimen along…

Microscope

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Microscopes are essential scientific instruments that extend the human ability to observe objects and structures far smaller than what the…

Differential Interference Contrast (DIC) Microscopy Vs Phase Contrast Microscopy

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Criteria Differential Interference Contrast (DIC) Microscopy Phase Contrast Microscopy Remarks Principle Converts phase shifts in light passing through a specimen…

Fluorescence Microscope

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A Fluorescence Microscope is a specialized optical microscope that uses fluorescence and phosphorescence to study properties of organic and inorganic…

Light Microscope

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A Light Microscope, also known as an optical microscope, is a fundamental scientific instrument that uses visible light and a…

Scanning Electron Microscope

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A Scanning Electron Microscope (SEM) is a sophisticated imaging device that creates detailed three-dimensional-appearing images of specimen surfaces by scanning…