Category: Lab Notes: Biotechniques

Volume Imaging Electron Microscopy

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Volume imaging electron microscopy refers to a collection of advanced electron microscopy (EM) techniques that enable the reconstruction of three-dimensional…

Oblique Plane Microscopy

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Oblique Plane Microscopy (OPM) is an innovative fluorescence imaging technique that enables rapid, three-dimensional imaging of living specimens by illuminating…

Two-Photon Microscopy

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Two-photon microscopy is an advanced fluorescence imaging technique that enables deep-tissue visualization with high spatial resolution and minimal photodamage. It…

Super-Resolution Microscopy

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Super-resolution microscopy encompasses a group of advanced optical imaging techniques that surpass the resolution limits of conventional light microscopy, enabling…

Widefield Microscopy

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Widefield microscopy is one of the most widely used and foundational techniques in optical microscopy. It involves illuminating the entire…

Laser Scanning Confocal Microscopy

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Laser scanning confocal microscopy (LSCM) is a powerful fluorescence imaging technique that enables the acquisition of high-resolution, optically sectioned images…

Laser Scanning Vs Spinning Disk Confocal Microscopy

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Criteria Laser Scanning Confocal Microscopy (LSCM) Spinning Disk Confocal Microscopy Remarks Principle Uses a single point laser beam scanned across…

Spinning Disk Confocal Microscopy

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Spinning disk confocal microscopy is an advanced imaging technique that enables rapid and high-resolution three-dimensional imaging of fluorescently labeled specimens,…

Acoustic Microscopy

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Acoustic microscopy is a non-destructive imaging technique that uses high-frequency sound waves—typically in the range of tens to hundreds of…

Multiphoton Microscopy

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Multiphoton microscopy (MPM) is an advanced fluorescence imaging technique that enables high-resolution, three-dimensional visualization of biological specimens, particularly deep within…

Compound Microscope

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A compound microscope is a fundamental optical instrument used to magnify small objects that are invisible to the naked eye….

Polarizing Microscopy

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Polarizing microscopy, also known as polarized light microscopy (PLM), is a specialized optical microscopy technique used to observe and analyze…

Scanning Tunneling Microscope (STM)

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A Scanning Tunneling Microscope (STM) is a revolutionary instrument that achieves atomic-scale imaging by utilizing quantum tunneling effects between a…

Atomic Force Microscope (AFM)

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An Atomic Force Microscope (AFM) is a sophisticated scanning probe microscope that achieves atomic-scale resolution by measuring forces between a…

Structured Illumination Light Sheet Microscopy (SILSM)

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Structured Illumination Light Sheet Microscopy (SILSM) is an advanced optical imaging technique that combines the strengths of two powerful microscopy…