Category: Lab Notes: Biotechniques
Single-Cell Chromatin Immunocleavage Sequencing (scChIC-seq)
Single-cell chromatin immunocleavage sequencing (scChIC-seq) is a powerful technique that combines the principles of chromatin profiling and single-cell resolution, enabling…
Electroencephalography (EEG)
Electroencephalography (EEG) is a non-invasive neurophysiological technique used to record the electrical activity of the brain through electrodes placed on…
Whole-Genome Sequencing
Whole-Genome Sequencing (WGS) is a comprehensive method used to determine the complete DNA sequence of an organism’s genome. This process…
Ion Torrent Sequencing
Ion Torrent sequencing is also known as Ion semiconductor sequencing. For more detailed information, please visit the article Ion Semiconductor…
Ion Semiconductor Sequencing
Ion semiconductor sequencing, also known as Ion Torrent sequencing, represents a significant advancement in next-generation sequencing technology. This method utilizes…
Alanine tRNA and the Birth of Sequencing: The Legacy of Robert W. Holley
In the annals of molecular biology, the sequencing of alanine transfer RNA (tRNA) from yeast stands as a monumental achievement….
Capillary Electrophoresis
Capillary electrophoresis (CE) is a powerful analytical technique used to separate ionic species based on their size-to-charge ratio. It is…
Automation of Sanger Sequencing Using Fluorescent Dideoxynucleotides and Capillary Electrophoresis
Sanger sequencing, developed by Frederick Sanger in the late 1970s, was the first widely adopted method for determining the nucleotide…
DNA Sequencer
A DNA sequencer is a laboratory instrument used to determine the precise order of nucleotides—adenine (A), thymine (T), cytosine (C),…
DNA Sequencing: Timeline of Key Events
DNA sequencing, the process of determining the precise order of nucleotides in a DNA molecule, has revolutionized biology, biotechnology, and…
Shotgun Sequencing
Shotgun sequencing is a powerful and widely used approach to DNA sequencing that involves randomly breaking a genome into numerous…
Maxam–Gilbert Sequencing
Maxam–Gilbert sequencing, also known as chemical sequencing, is one of the first DNA sequencing methods ever developed. Introduced in 1977…
First-Generation Sequencing
First-Generation Sequencing encompasses the earliest set of DNA sequencing technologies developed in the 1970s and 1980s, which provided the first…
Third-Generation Sequencing (TGS)
Third-Generation Sequencing (TGS) refers to a set of advanced DNA sequencing technologies that enable the direct reading of long DNA…
DNA-Based Tools
DNA-based tools have transformed the landscape of biotechnology, offering unprecedented precision, scalability, and versatility across fields such as medicine, agriculture,…