OVERVIEW | |
| Organism | Human (Homo sapiens), 54 years caucasian female (Ref: atcc) |
| Source/Tissue of origin | Stomach, Gastric adenocarcinoma (Ref: atcc) |
| Year of isolation | |
| Morphology | Epithelial-like (Ref: atcc) |
| Cell type | Gastric epithelial |
| Growth mode | Adherent, monolayer |
| Doubling time | ≈ 20 hrs (Ref: atcc) |
| Life span | Infinite, continuous culture |
| Karyotype | Hyperdiploid (Ref: atcc) Modal chromosome number: 49 (in 60% of cells) (Ref: atcc) Polyploidy rate: 3.6%. (Ref: atcc) |
| Transformed | Yes, in nude mice (estrogen-dependent) (Ref: Osborne et al., 1985) |
| Tumorigenic | ? |
| Drug resistance | |
| Biosafety Level | BSL 1 (Ref: atcc) (It may differ in your country, follow the guidelines of your institute/country) |
| Gene Expression | |
| Products | |
| Applications | 3D cell culture (Ref: atcc) Suitable transfection host (Ref: atcc) |
| Remarks | |
| Keywords | A human mammary epithelial adenocarcinoma cell line |
| Related cell lines | |
Culture maintenance | |
| Culture medium* | DMEM + 10% FBS |
| Culture conditions | Humidified incubator, 95% air + 5% CO2, 37°C |
| Subculturing procedure | Trypsin-EDTA |
Culture storage | |
| Cryo-medium | Complete growth medium + 5% (v/v) (Ref: atcc) |
| Storage conditions | Vapor phase of liquid nitrogen (Ref: atcc) |
REFERENCES
- https://www.atcc.org/products/crl-1739
- Sutherland et al., 1983. Cell proliferation kinetics of MCF-7 human mammary carcinoma cells in culture and effects of tamoxifen on exponentially growing and plateau-phase cells. Cancer Res. 43(9), 3998-4006. PMID-6871841
- Osborne et al., 1985. Effect of estrogens and antiestrogens on growth of human breast cancer cells in athymic nude mice. Cancer Res. 45(2), 584-90. PMID-3967234; Full-Text Links: Silverchair
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