Posted in Database: Cell Line

Cell Line: SK-MEL-24

   October 16, 2024  Leave a Comment on Cell Line: SK-MEL-24

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  • Cell Line: SK-MEL-24
  • Synonyms:
    • SK-MEL-24
    • SKMEL-24
    • SKMEL24
    • SKMel24
    • SK-Mel24
    • SK Mel 24
    • Sk-Mel-24
  • Culture Type: Cell line
  • Keywords:  Human Melanoma Cell Line
  • Description: Established from the skin of a 67-year-old, Caucasian male patient with melanoma
  • Origin:
    • Organism:
      • Species: Human (Homo sapiens)
      • Ethnicity: Caucasian
      • Age: 67 years
      • Gender: Male
    • Tissue: Skin
    • Disease: Melanoma
    • Primary/Metastasis: Metastatic (DepMap)
    • Collection site: Lymph node (ATCC)
    • Year of origin:-
    • Country of Origin:-
  • Cell line features:
    • Cell Type:-
    • Morphology: Stellate
    • Growth mode: Adherent, monolayer
    • Life span: Infinite (continuous culture)
    • Doubling time: –
    • Karyotype:-
    • Microsatellite instability: Stable
    • Special features:-
    • Product:-
    • Soft agar colony formation assay:-
    • Tumorigenicity: Yes, tumorigenic in nude mice, forms malignant melanoma
    • Gene expression:-
    • Sequence variations: BRAF (V600E mutation, Oncogenic, Gain-of-function, Heterozygous)
    • For more information, please visit:
      • https://depmap.org/portal/cell_line/ACH-000822?tab=mutations
      • https://depmap.org/portal/cell_line/ACH-000822?tab=fusions
  • Handling and maintenance:
    • Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
    • Complete culture medium: Eagle’s Minimum Essential Medium+  15% FBS (ATCC)
    • Culture conditions: 
      • Temperature: 37°C incubator
      • Atmosphere: 95% Air, 5% CO2
    • Subculturing:
      • Subculturing method: Enzymatic dissociation
      • Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution (ATCC)
      • Harvesting time:
      • Seeding density:-
      • Split ratio: 1:2 to 1:4 (ATCC)
      • Split frequency:-
      • Medium Renewal: 2 to 3 times per week (ATCC)
    • Cryopreservation:
      • Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
      • Storage conditions: Vapor phase of liquid nitrogen
      • Cell suspension (cell density):-
      • Volume per vial: 1 ml
  • Applications: 
    • 3D cell culture
    • cancer research
  • Cell Line Availability: The cell line can be obtained from the following sources.
  • References
  • Images:-
  • Abbreviations
    • BSL (Biosafety level)
    • FBS (Fetal Bovine Serum)
    • DMSO (Dimethyl sulfoxide)
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    • Please let us know if you find any mistakes. Your comments are welcome in the comment box.
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