- Cell Line: SK-MEL-28
- Synonyms:
- SK-MEL-28
- SKMEL-28
- SKMEL28
- SKMel28
- SK-Mel28
- SK Mel 28
- Sk-Mel-28
- Culture Type: Cell line
- Keywords: Human Melanoma Cell Line
- Description: Established from the skin of a 51-year-old, male patient with malignant melanoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Ethnicity: Caucasian
- Age: 51 years
- Gender: Male
- Species: Human (Homo sapiens)
- Tissue: Skin
- Disease:
- Malignant Melanoma (ATCC)
- Cutaneous melanoma (Cellosaurus)
- Primary/Metastasis: Primary (DepMap)
- Collection site: Skin (DepMap)
- Year of origin:-
- Country of Origin:-
- Organism:
- Cell line features:
- Cell Type: Melanocyte
- Morphology: Polygonal cells
- Growth mode: Adherent, monolayer
- Life span: Infinite (continuous culture)
- Doubling time:
- 27 hours (Cellosaurus)
- 35.1 hours (Cellosaurus)
- Karyotype: Modal number = 90, range = 81 to 96, hypotetraploid human cell line
- Microsatellite instability: Stable
- Special features: Lightly pigmented (Cellosaurus)
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity: Tumorigenic in nude mice
- Gene expression:-
- Sequence variations:
- PTEN (T167A mutation, Oncogenic, Loss-of-function, Homozygous)
- CDK4 (R24C mutation, Oncogenic, Gain-of-function, Heterozygous)
- TP53 (L145R mutation, Oncogenic, Loss-of-function, Homozygous)
- BRAF (V600E mutation, Oncogenic, Gain-of-function, Homozygous)
- For more information, please visit:
- https://depmap.org/portal/cell_line/ACH-000615?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000615?tab=fusions
- Cell Type: Melanocyte
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Eagle’s Minimum Essential Medium+ 10% FBS (ATCC)
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Applications:
- 3D cell culture
- Cancer research
- Toxicology
- High-throughput screening
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- Cytion: Product link
- BCRJ: Product link
- References
- Images:
- SK-MEL-28 cell line image link 1 (source: Cytion)
- SK-MEL-28 cell line image link 2 (source: Cytion)
- SK-MEL-28 cell line image link 3 (source: Cytion)
- SK-MEL-28 cell line image link 4 (source: Cytion)
- SK-MEL-28 cell line image link 5 (source: Cytion)
- SK-MEL-28 cell line image link 6 (source: Cytion)
- SK-MEL-28 cell line image link 7 (source: DepMap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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