- Cell Line: SUP-B15
- Synonyms:
- SUP-B15
- SUPB-15
- SUPB15
- Sup-B15
- Culture Type: Cell line
- Keywords: Human B Lymphoblast Cell Line
- Description: Established from the bone marrow of a White, 8-year-old, male patient with acute lymphoblastic leukemia (ALL). (ATCC)
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age:
- 8 years (ATCC)
- 9 year (Cellosaurus, DepMap, DSMZ)
- Ethnicity: Caucasian
- Gender: Male
- Tissue: Bone Marrow/Lymphoid
- Disease: Acute lymphoblastic leukemia (ALL)
- Primary/Metastasis: Primary (DepMap)
- Collection site: Bone marrow
- Year of origin: 1984 (DSMZ)
- Country of Origin:-
- Organism:
- Cell line features:
- Cell Type: B lymphoblast
- Morphology: Lymphoblast-like; single, round cells
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Doubling time:
- Karyotype: Human pseudodiploid karyotype
- Microsatellite instability: Stable (Cellosaurus)
- Special features: The cells express multiple B lineage markers, but do not express T cell markers.
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000059?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000059?tab=fusions
- For information, please visit
- Cell Type: B lymphoblast
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC, DSMZ) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time: 2 x 106 cells/mL
- Seeding density: 5 x 105 viable cells/mL
- Recommended split ratio: 1:2 to 1:4
- Medium Renewal: 2 to 3 times per week
- Subculturing method: Dilution
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Applications:
- 3D cell culture
- Immunology
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- References
- Images:
- SUP-B15 cell line image link 1 (Source: ATCC)
- SUP-B15 cell line image link 2 (Source: DSMZ)
- SUP-B15 cell line image link 3 (Source: DSMZ)
- SUP-B15 cell line image link 4 (Source: DSMZ)
- SUP-B15 cell line image link 5 (Source: DSMZ)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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