- Cell Line: SVCT-MI2
- Synonyms:
- SVCT-MI2
- Culture Type: Cell line
- Keywords: Human Breast Epithelial Transformed Cell Line
- Description: Established by transforming breast epithelial cells with SV40
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Ethnicity:-
- Age:-
- Gender: Female
- Species: Human (Homo sapiens)
- Tissue: Breast
- Disease:-
- Primary/Metastasis:-
- Collection site: Breast
- Year of origin: 1987
- Country of Origin:-
- Organism:
- Cell line features:
- Cell Type:-
- Morphology:-
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Doubling time:-
- Karyotype:-
- Microsatellite instability:-
- Special features:-
- Product:-
- Soft agar colony formation assay: Yes, form colonies
- Tumorigenicity: Tumorigenic in nude mice
- Gene expression:-
- Sequence variations:
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 2 (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI 1640 or DMEM + 2mM Glutamine + 10 µg / ml insulin + 5 µg/ml hydrocortisone + 10% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 95% Air, 5% CO2
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: Trypsin- EDTA solution
- Harvesting time: sub-confluent cultures (70-80%)
- Seeding density:-
- Split ratio: 1:4 to 1:10
- Split frequency:-
- Medium Renewal:-
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO??
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): 1×106 cells/ml
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 20% (v/v) FBS + 10% (v/v) DMSO??
- Applications:
- Cancer research
- Drug Development
- Cell Line Availability: The cell line can be obtained from the following sources.
- Sigma-Aldrich: Product link
- Cancertools: Product link
- Ximbio: Product link
- References
- Images:
- SVCT-MI2 cell line image link 1 (Source: cancertools)
- SVCT-MI2 cell line image link 2 (Source: ximbio)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
