- Cell Line: SW-13
- Synonyms:
- SW-13
- SW 13
- Culture Type: Cell line
- Keywords: Human Adrenal Cortex Carcinoma Cell Line
- Description: Established from the adrenal gland and cortex of a Caucasian, 55-year-old, female patient with Adrenal Cortex Carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 55 years
- Ethnicity: Caucasian
- Gender: Female
- Tissue (origin): Adrenal cortex
- Disease: Adrenal Cortex Carcinoma
- Primary/Metastasis: Primary
- Collection site:-
- Year of origin: 1971
- Organism:
- Cell line features:
- Cell Type: Epithelial
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: Modal number = 63, Range = 45 to 65
- Doubling time:-
- Microsatellite instability: stable
- Special features: Electron microscopic studies show many bulb gap junctions (BGJ)
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- TP53 (H193Y mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-001401?tab=mutations
- https://depmap.org/portal/cell_line/ACH-001401?tab=fusions
- TP53 (H193Y mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Leibovitz’s L-15 Medium + 10% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 100% Air
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.03 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:3 to 1:8
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% (v/v) DMSO
- Complete growth medium + 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product links
- Cytion: Product links
- References
- Images:
- SW-13 cell line image link (Source: DepMap)
- SW-13 cell line image link 1 (Source: Cytion)
- SW-13 cell line image link 2 (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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