T-225 flasks are available from different suppliers in both ventilated and non-ventilated formats. They are useful when you need a large number of cells.
| Specifications | Optimum | Range |
| Available area for cell growth | 225 cm2 | NA |
| Volume of trypsin (or dissociating agent) | 10 ml | 7 – 18 ml |
| Volume of culture medium | 45 ml | 40 – 60 ml |
| Cell number at the time of seeding * | 4.5 x 106 cells Ɨ | 3.6 – 7.5 x 106 cells |
| Cell number at 100% confluency * | 26 x 106 cells | 26 – 29 x 106 cells # |
| * for the HeLa cell line Ɨ Correspond to seeding density 2 x 104 cells/cm2 (Ref) # Overconfluent culture after this stage | ||
